HEK293T cells were cotransfected with pCF-BBtr, the empty vector pcDNA, or pcfeA3Z2b-HA, and increasing amounts of plasmids encoding either wt or mutant Bet (Figure。
The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。
FeFAB assay indicated that the culture supernatants collected from the infected and drug-pretreated cells contained infectious FFV virions, which induce expression of the β-galactosidase in the ...
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